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1969). Nucleoli—the cellular site for ribosome production. ), pp. 620-636. North Holland Publishing, Amsterdam. 26 MARGERY G. ORD and LLOYD A. STOCKEN Rich, A. (1960). A hybrid heHx containing both deoxyribose and ribose polynucleotides and its relation to the transfer of information between the nucleic acids. Proc. Natl. Acad. Sci. USA 46,1044-1053. A. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487-491. Sakaba, K. & Okazaki, R. (1966).

Coli plasmid that contained phage DNA and the complete E. coli galactose operon. While they were able to demonstrate, using physical means, that chimeric molecules had been formed, the biological activity of these chimeric molecules could not be tested—the EcoKl site used to linearize the circular SV40 genome cut a gene essential for replication. Further work on SV40 stopped because of concerns about the safety of using DNA from an oncogenic virus as a vector. These worries led to the Berg letter and to the great recombinant DNA debates of the 1970s (Rogers, 1977; Watson and Tooze, 1981).

A. (1957). Virus reconstitution: Combination of protein and nucleic acid from different strains. Biochim. Biophys. Acta 24, 540-548. E. G. (1953). Molecular configuration of sodium thymonucleate. Nature, Lond. 171,740-741. G. L. (1969). Formation and detection of RNA—DNA hybrid molecules in cytological preparations. Proc. Natl. Acad. Sci. USA 63, 378-383. Gamow, G. (1954). Possible relation between DNA and protein structure. Nature 173, 318. L. (1975). DNA replication. Annu. Rev. Biochem. 44,45-78.

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Classical Theory of Radiating Electrons by P. A. M. DIRAC

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