Advances in cell culture. Volume 1 - download pdf or read online

By Karl Maramorosch

ISBN-10: 0120079011

ISBN-13: 9780120079018

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Extra info for Advances in cell culture. Volume 1

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While monoacetylated histone H4 is present only to a small extent in control HeLa and Friend erythroleukemia cells, Riggs and her associates (1977) have demonstrated t h a t multiacetylated forms of H4 predominate in sodium butyrate-treated cells. In addition, a broadening of the H3 histone band with an increase of slower moving components and a sharp increase in the relative amounts of H I species have been concomitantly observed. All of these metabolic effects are readily reversible within 24 hours after removal of sodium butyrate from the cells.

1976). Calcium apparently plays a role in the assembly of microfilaments and Henneberry and associates (1975) have shown t h a t relatively low concentrations of the ionophore A23187, which is known to alter intracellular calcium levels, pre- 36 RODY P. COX AND DEBRA GENDUSO DAY FIG. 4. Morphological effects of sodium butyrate on HeLa 65 cells and the alterations mediated by cytochalasin-B and colcemid. (a) HeLa 65 cells grown in medium without additions (control). Cells are rounded and grow as adherent epithelial colonies, (b) HeLa 65 cells grown in medium with sodium butyrate (1 mM) for 48 hours.

Surprisingly, low levels of FSH were found in HeLa cell cultures and the level of hormone production was increased by butyrate as shown in Table V (Ghosh and Cox, 1977). The unexpected finding of FSH synthesis by a HeLa cell line derived from cervix required a careful validation of the radioimmunoassay in view of the known secretion of a subunits and HCG. Two different antisera were used in this investigation. One was prepared against highly purified h u m a n pituitary ß subunit of FSH and the other against the complete hormone.

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Advances in cell culture. Volume 1 by Karl Maramorosch


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